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. 2012 May 2;7(5):e35231. doi: 10.1371/journal.pone.0035231

Figure 8. Exon splice junction sequences in the murine and human VEGF-A genes.

Figure 8

Exon junction sequences are shown for the murine VEGF-A gene (A) and human VEGF-A gene (B), highlighting the sequence TGCAG, which exists at the end of exons 3, 4 & 7 (in-frame stop codons are asterisked). Since any VEGF164b/188b reverse primer sequence will contain sequence complementary to these bases, this highlights the possibility of obtaining truncated VEGF PCR products. The sequence CAG also occurs within the 3′UTR, immediately prior to the start of the predicted exon8b sequence (shown in italics), demonstrating the need for VEGF164b/18b isoform-specific primers to contain a minimum of four bases that span the exon8b/exon7 junction.