Figure 1. Tetraspanin-enriched HIV-1 positive compartments in infected MDMs are not accessible to the external environment.

Human MDMs were infected with VSV-G-pseudotyped HIV-1. 8 days post infection, cells were fixed and immunolabeled for tetraspanins (red, anti-CD81, anti-CD9, or anti-CD63) without cell permeabilization, followed by permeabilization and immunolabeling for HIV-1 Gag (green, anti-MA). Image acquisition was performed with an Improvision/Perkin Elmer spinning disc confocal fluorescence microscope. Bars represent 16 µm.