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. 2011 Dec 13;2:75. doi: 10.3389/fimmu.2011.00075

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Copyright © 2011 Keskin, Reinhold, Lee, Zhang, Lank, O’Connor, Berkowitz, Brusic, Kim and Reinherz.

This is an open-access article distributed under the terms of the Creative Commons Attribution Non Commercial License, which permits non-commercial use, distribution, and reproduction in other forums, provided the original authors and source are credited.

PMC Copyright notice

Multiple alignment of HPV-16 E7 protein sequences determined from tumor DNA sequencing. HPV-16 E7 DNA sequences were PCR-amplified from total tumor DNA and sequenced. DNA sequences were translated to and protein sequences aligned for comparison. Two HPV-16 E7 sequence variants are present in the analyzed tumor samples: N₂₉ in KTS2, KTS3, and KTS6 marked as red, and S₂₉ in the remaining samples. The E7 _11–19 YMLDLQPET T-cell epitope highlighted as yellow was conserved in all samples. Results are representative of two independent sequencing reactions performed on nine different tumor DNA samples.