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Overall HLA-A2 peptide recovery is marked by a “hump” in the m/z range from 450 to 550 due to doubly charged peptide molecular ions relative to singly charged background ions (see Figure A9 and related discussion). This figure illustrates good MHC I peptide recovery in all four samples shown. (A) E711–19 positive sample KTS5; (B) E711–19 positive sample KTS7; (C) E711–19 negative sample KTS8; (D) E711–19 negative sample KTS9.
