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(A) BB7.2 pulldown and acid extracted peptide pool from TAP-deficient T2 cells showing prominent GILT signal peptides at m/z 449.78 (LLDVPTAAV2H+), m/z 460.77 (LLDVPTAAVNa+H+), and m/z 605.88 (LLLDVPTAAVQA2H+). (B) BB7.2 pulldown and acid extracted peptide pool from TAP-sufficient T1 cells. The m/z 449.78 peak is still primarily the GILT signal peptide LLDVPTAAV2H+ but a complex set of other doubly charged peptides cover the m/z range 420–620.
