Table 1. Oligonucleotide primers used for RT-PCR, RACE and diagnostic PCR.
Primer name | Sequence (5′ to 3′)* | Description |
258.RYRF13 | TGGTGGACNGTNCAYCCNGC (WWTVHPA) | RT-PCR product P1 |
127.RYRR1 | TCCATYTTNCCYTCYTCRTG (HEEGKMD) | |
279.H2.2F2 | TGGGCAAAGTGGAGGAGAAG | RT-PCR product P2 |
280.A1.1R1 | CTCACTGTCTCCGAAGCCGT | |
139.RYRF7 | GARAAYACNCAYAAYYTNTGG (ENTHNLW) | RT-PCR product P3 |
141.RYRR7 | TCCCARTTNGCYTTYTCCAT(MEKANWE) | |
303.A1.1F1 | GATACTCCACCTTGCCTG | RT-PCR product P4 |
143.RYRR8 | TCRTTYTTRCANACYTCCAT(MEVCKNE) | |
327.K3.9F1: | TGCGGCAAGGCTTCTATGAC | RT-PCR product P5 |
302.J1.6R1 | CCATCAGACTGGAGTAAGCG | |
290.RYRF14 | GCNATGTTYGAYCAYTTYGA(AMFDHFD) | RT-PCR product P6 |
257.RYRR13 | TCNCCRTTNACCCANACRCA(CVWVNGE) | |
296.J1.6F1 | GCAACTCGGAGCTGGTAG | RT-PCR product P7 |
239.A2.7R | CCCGTACACCCAACCATTCT | |
146.RYRF10 | GARCAYTAYCAYGAYGCNTGG(EHYHDAW) | RT-PCR product P8 |
147.RYRR10 | GCNACCATYTCYTTYTCYTT(KEKEMVA) | |
238.A2.7F1 | CGCATCGCAACTACTTCATA | RT-PCR product P9 |
235.A3.7R1 | CCACAACTGTAGTGACTCTGCG | |
148.RYRF11 | CCNTGGATGACNMGNATHGC(PWMTRIA) | RT-PCR product P10 |
149.RYRR11 | TGYTGNGGRTGRTCDATCAT(MIDHPQQ) | |
237.A3.7F2 | GCCAAGATCATAGACGACACG | RT-PCR product P11 |
234.47.5R1 | CCAAAGCCTGCTGGTTCTGT | |
132.RYRF4 | ATGGAYTTYTAYTGGCAYTA (MDFYWHY) | RT-PCR product P12 |
133.RYRR4 | GGYTCRTTNGGCATRTGYTC(EHMPNEP) | |
153.RYRSF1 | GGACTACATCGGGTTCTGCG | RT-PCR product P13 |
155.RYRSR2 | GGTCTTCTCCATGCCCAGCA | |
134.RYRF5 | GTNAAYTAYTGGGAYAARTT (VNYWDKF) | RT-PCR product P14 |
136.RYRR5 | CATRTTCCANACRTANGTYTC(ETYVWNM) | |
276.H2.2R1 | TGGAAGAACCTCAGCACATCGC | 5′ -RACE product R5 |
277.H2.2R2 | ACACTAAGATCAAGTCGTCTCCCAC | |
201.48F1 | CAACAAGAACTGCCACGACA | 3′ -RACE product R3 |
202.48F2 | GAGCATAACTTGGCGAACTACA | |
371.CmRyRF1 | GTCAGTTCCGGGAAATGGTA | diagnostic PCR for exon a |
372.CmRyRR1 | CCCACTGCTTGCCAAACGAC | |
373.CmRyRF2 | CAGTTTCGGGCAGCAGTTCA | diagnostic PCR for exon b |
374.CmRyRR2 | ATCCTGGTATCTGGCATTTC | |
375.CmRyRF3 | AACAACGACCTCAACACCAT | diagnostic PCR for the absence of exon c |
376.CmRyRR3 | GTGGCTGAATCCGTACCACC | |
377.CmRyRF4 | TCGACCACCAGATGTCGTGA | diagnostic PCR for the presence of exon c |
378.CmRyRR4 | TATCGGTGAGGAGGTCGTAG |
Corresponding amino acid sequences for degenerate primers were shown in parentheses.