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. 2012 May 2;7(5):e34719. doi: 10.1371/journal.pone.0034719

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A. Left: Diagram of Brd4 deletions. BDI and BDII represent bromodomains, ET stands for the ET domain. ΔET and ΔC are devoid of the internal C-terminal region, but contain the extreme C-terminal 83 amino acids. Right: A summary of nocodazole induced Brd4 release. + indicates retention of Brd4 on mitotic chromosomes, while– indicates Brd4 release after nocodazole treatment. B. Cells expressing free GFP, GFP-full length (FL) Brd4 or indicated deletions were treated with nocodazole (top panel) or vehicle alone (bottom panel) for 4 h and localization of GFP and chromosomes (DNA) was examined by microscopy. Approximately 200 mitotic cells were inspected for each deletion construct. After nocodazole treatment, more than 90% cells expressing ΔET&C and ΔC retained GFP signals on chromosomes, while less than 5% of full length Brd4 did so. Bromodomain deletions (ΔI, ΔII, and ΔI&II) and Free GFP did not localize to chromosomes, irrespective of drug treatment.