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. 2001 May 15;98(11):6068–6073. doi: 10.1073/pnas.111139698

Figure 2.

Figure 2

Binding of NPG to RSO membrane vesicles. Binding of [3H]NPG to nonenergized RSO vesicles was assayed by flow dialysis as described in Experimental Procedures. An aliquot of RSO vesicles (250 μl) in 0.1 M KPi (pH 7.5) at a protein concentration of 32 mg/ml was placed in the upper chamber. [3H]NPG (4 μl; 0.95 mCi/mmol; ≈4 × 106 cpm) was added to a final concentration of 16 μM at fraction 1. As indicated by the arrow, 2.5 μl of TDG was added to the upper chamber to a final concentration of 10 mM. For clarity, curves are displayed in decreasing order; however, the actual levels of radioactivity in the dialysate were identical in fractions 1 through 9 in each case (i.e., the curves should be superimposed).