Fig. 4.
Giardia lamblia NIMA-related serine/threonine kinase 2 (GlNek2) localizes to the spindle poles and spindle apparatus during the cell cycle. Trophozoites expressing HA-tagged GlNek2-wild type (WT) under the control of its own promoter were co-stained with antibodies against the HA-tag and phosphorylated Aurora Kinase (pAK). DAPI illustrates the localization of the nuclei. The arrowheads indicate the anterior axonemes/paraflagellar rods. The arrow indicates the re-organized basal bodies. The corresponding differential interference contrast (DIC) image is shown. Bar = 10 μm.