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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Int J Parasitol. 2012 Mar 13;42(4):411–419. doi: 10.1016/j.ijpara.2012.03.001

Fig. 5.

Fig. 5

Effect of Giardia lamblia NIMA-related serine/threonine kinases, GlNek1 and GlNek2, on growth and cytokinesis. (A) Equal numbers of cells over-expressing wild type (WT) or mutant (KM) GlNek 1 or GlNek2 were inoculated at time 0 and cell growth was determined after 24 and 48 h. * P < 0.001, ** P < 0.0001. (B) Immunofluorescence analyses of GlNek-OCT-KM trophozoites with antibodies against the HA-tag and phosphorylated aurora kinase (pAK) show a large proportion of cells with an intact pAK labeled parental disk during cytokinesis (“Stalled Cytokinesis”), indicated by an arrow. Normally, cells in cytokinesis (“Progressing Cytokinesis”) display the typical pAK stained and pontoon shaped disassembling parental disk. The corresponding differential interference contrast (DIC) image is shown. (C) Bar graph represents the percentage of cells in cytokinesis per strain. ** P = 0.0001. Simple one-way ANOVA was used to determine statistical significances.