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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Int J Parasitol. 2012 Mar 13;42(4):411–419. doi: 10.1016/j.ijpara.2012.03.001

Fig. 6.

Fig. 6

Giardia lamblia NIMA-related serine/threonine kinases, GlNek1 and GlNek2, in the Giardia life cycle. (A) Cell lines expressing GlNek1-HA or GlNek2-HA under their own promoter were encysted and excysted. Total protein levels of GlNek 1 and GlNek2 from vegetative trophozoites (Veg), 21 h or 42 h encysting trophozoites, cysts and Stage 1 (S1) and Stage 2 (S2) excysting cells were compared by Western blot with the anti-HA-HRP (horseradish peroxidase) antibody. Taglin was used as a protein loading control. The graph shows the band intensities of GlNek1 and GlNek2 relative to vegetative cells (Veg). (B) Equal numbers of cysts from cell lines over-expressing wild type (WT) or mutant (KM) GlNek1 or GlNek2 were excysted and excyzoites were counted. Bars represent the mean number of excyzoites from three independent experiments from each cell line relative to the empty vector control. * P < 0.05, ** P < 0.005. Simple one-way ANOVA was used to determine statistical significance.