Figure 1.
Characteristics of the mast cell synapse. (A) Confocal section demonstrating synapse formed by a MC/9 mast cell primed with α-DNP IgEA488 after 20 min on a 25 mol% DNP-lipid bilayer. (B) Structure of the actin cytoskeleton in a RBL cell primed with α-DNP IgEA488 after 20 min on a 25 mol% DNP-lipid bilayer. Confocal section of cells fixed with paraformaldehyde and then labeled with rhodamine phalloidin to visualize actin. (C) Total internal reflection fluorescence microscopy (TIRFm) image of an RBL cell repleted with FITC-conjugated cholesterol and primed with α-DNP IgEA555 after 20 min on a 25 mol% DNP-lipid bilayer. (D) Confocal section of RBLs co-primed with equal concentrations of α-DNP IgEA488 and α-dansyl IgEA555 and settled onto 25 mol% DNP-lipid bilayers after 20 min. (E) Confocal sections of RBLs primed with α-DNP IgEA555 and pretreated with either Latrunculin B (upper row) or PP2 (lower row) settled on 25 mol% DNP-lipid bilayers for 20 min. Cells were paraformaldehyde fixed and stained with A488 phalloidin. All images have been brightness and contrast enhanced. Scale bars are 5 μm.