Figure 2.

Interactions between mast cells and dendritic cells. (A) Immunohistochemistry of normal human intestinal and colon tissue double stained for mast cell tryptase (brown) and CD209 (DC-SIGN, pink). Arrows within blown up sections indicate regions where direct contact between the two cell types is observed. (B) Live cell, confocal imaging of a mouse bone marrow-derived mast cell primed with α-DNP IgE_A488 (green) directly interacting with a mouse bone marrow-derived dendritic cell after activation with DNP–BSA. Arrows indicate where mast cell internalized material has been transferred to the dendritic cell. All images have been brightness and contrast enhanced.