Figure 5.
The CD8+ T cell response in MOG−/− mice focuses on an epitope with high affinity for Db. (A) RMA-s cells were cultured with the indicated peptides and expression of Db assessed by flow cytometry (B) shows the geometric mean fluorescence (MFI). Mice were immunized with 100 μg pMOG in CFA. Ten days later, CD8+ T cell lines were derived by restimulation of CD4-depleted LN cells with pMOG. After five rounds of stimulation, (C) T cells were tested for recall responses to pMOG or (D) to the indicated peptides. (E) A MOG−/− CD8+ T cell line was cultured with dose ranges of the indicated peptides. Each experiment shown is one of two/three experiments giving consistent results.