Figure 6.

CD8+ T cells are unable to induce EAE. Donor mice were immunized with 100 μg pMOG in CFA. Ten days later, LN cells were restimulated with pMOG for 3 days in the presence of IL-12 and IL-18. Cells were analyzed pre-transfer for CD4+ and CD8+ T cells within the donor population (A). Unsorted cells were injected i.v. into WT host mice (B). CD4+ and CD8+ T cells were purified by FACS and injected into WT hosts (C). CD8+ T cells (3 × 10⁶) were transferred alongside 1 × 10⁶ unsorted cells (D) or 4 × l0⁶ injected into mice with active disease (immunized 13 days previously) (E). Each experiment shown in one of two/three experiments giving consistent results; three–five mice per group were used. Figure (E) is a pool of three experiments with eight–nine mice per group.