Figure 10.

Truncated Sp185/333 proteins are present in the coelomic fluid. Enlarged regions of three different two dimensional Western blots of coelomic fluid proteins from the same sea urchin were analyzed with different anti-Sp185/333 sera; anti-66, anti-68, or anti-71. Anti-66 recognizes AHAQRDFNERRGKENDTER from element 1; anti-68 recognizes GGRRGDGEEETDAAQQIGDGLC from element 7; anti-71 recognizes TEEGSPRRDGQRRPYGNR from element 25 (see Figure 6A for element positions). Decreasing numbers of spots in blots analyzed with antisera that recognize peptides in more C-terminal regions of the proteins suggests that many are either truncated or have missense sequence toward the C-terminus. Reprinted from Dheilly et al. (2009) with permission from the American Association of Immunologists, Inc., copyright 2009.