Figure 11.

A single sea urchin can have as many as 264 spots that are Sp185/333⁺. Coelomic fluid proteins (200 μg) from sea urchin 12 (see Figure 12) were separated by 2D electrophoresis and transferred to a filter. The filter was immunostained with an equal mixture of the three anti-Sp185/333 sera (see legend to Figure 10) and exposed to autoradiographic film for 1, 5, or 10 min. The different exposures were merged to give a final composite image. Isoelectric points (pI) are shown at the top and the molecular weight standards (kDa) are shown to the left. Reprinted from Dheilly et al. (2009) with permission from the American Association of Immunologists, Inc., copyright 2009.