Figure 13.

Native Sp185/333 proteins bind Vibrio diazotrophicus (Vd), a gram negative marine bacterial species. Whole coelomic fluid (wCF) lysate from a sea urchin was incubated with Vd. Bacteria were pelleted, washed, and analyzed by Western blot using equal amounts of all three anti-Sp185/333 sera (see legend for Figure 10). wCF and Vd alone are shown for comparison. Lane 1, wCF; lane 2, wCF proteins bound to Vd; lane 3, Vd. Protein standard is to the left. Unpublished figure provided by Catherine Schrankel.