Figure 14.
A recombinant Sp185/333 protein (rSp0032) binds Vibrio diazotrophicus (Vd). Biotinylated rSp0032 incubated with 109 Vd cells and post-labeled with Neutravidin–FITC shows increased binding with increased protein concentration within the gate area (R2). Binding plateaus at about 400 mM of rSp0032 indicating the saturation point. MFI, mean fluorescence intensity. Results from flow cytometry (inserted images) show fluorescence (X-axis; fluorescent events measuring FITC) associated with bacterial cells (Y-axis; side scatter or cell counts) for increasing concentrations of rSp0032. Unpublished figure provided by Catherine Schrankel.