Figure 8.

Six Sp185/333 genes are linked on the 7096 BAC insert. The finished-level assembly of the region containing the Sp185/333 genes was experimentally confirmed by PCR, pulsed field gel electrophoresis, AseI digests, and subclone sequences. The six Sp185/333 genes include one gene with the A2 element pattern (red), one B8 gene (orange), three D1 genes (yellow, green, blue), and one E2 gene (purple; see Figure for element patterns). All are located near the 3′ end of the BAC insert. Gene orientations are indicated and spacing is to scale unless otherwise noted. GA microsatellites flank each gene and GAT microsatellites flank segmental duplications that are positioned on 5′ side of B8 and include the three D1 genes. This is Figure 4 from Miller et al. (2010) reproduced with permission from BioMed Central.