Table 1.

Mass spectrometric identification of native Sp185/333 proteins from coelomic fluid^*.

Sp185/333 sequence	Element1
DFNERREKENDTERGQGGFGGRPGGMQMGGP	1–2
RGRGQGRFGGRPGGMQMGGPRQDGGPMG	1–2–3
GQGGFGGRPGGMQ[M/T]GG[P/L]R	2
RFDGPESGAPQM[E/G]GRRQNGVPMGGR	4
MGGRnstnPeFGGSRPDGAG2	6–7
(Overlap with above)FGGSRPDGAGGRPFFGQGGR2	6–7
RGDGEEETDAAQQIGDGLGGPGQFDG[P/H]GR	7–8–9–10
PQTDQRNNRLVSATKAAMRM3	13–14
MAVLTLATMAATTSIIIATTQKVTK4	14–15
KPFGDHPFGR	23
adVVEIAVNEEDVN2	25b

*Amino acid sequences of peptides identified by LC–MS/MS from gel slices after one dimensional separation of coelomic fluid proteins from three different sea urchins as reported in Dheilly et al. (2009). Modified from Table 1 in Dheilly et al. (2009).

¹Element in which the sequence is present. For description of elements, see Buckley and Smith (2007), Terwilliger et al. (2007), and Figure 6A.

²Exact matches were not found from the translated cDNAs reported by Terwilliger et al., 2007, see Supplemental Table 2). Lower case letters indicate mismatched amino acids between proteomic result and deduced sequence.

³Missense sequence matches to the amino acid sequence predicted from one cDNA (GenBank accession number; EF065834) reported by Terwilliger et al. (2007).

⁴Missense sequence matches to amino acid sequences predicted from five cDNAs (GenBank accession numbers; EF065834, EF065907, EF066020, EF066222, EF066214) reported by Terwilliger et al. (2007).