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. 2012 Apr 25;3:85. doi: 10.3389/fimmu.2012.00085

Figure 1.

Figure 1

TH17 cells signature cytokines, IL-17A and IL-17F, mRNA transcripts are increased in the liver during a primary P. chabaudi infection. C57BL/6 mice were infected with 105 P. chabaudi and sacrificed post-infection at the time points indicated. Spleen and liver mononuclear cells were harvested from naïve and infected mice and analyzed by real-time PCR. (A) Representative course of infection in C57BL/6 mice. IFN-γ, IL-17A, IL-17F, and IL-22 mRNA levels were determined in naïve and infected splenocytes (B) and liver mononuclear cells (C). Data are normalized to Ubiquitin expression. Test samples were expressed as the fold increase of gene expression compared with expression in normal uninfected mice (mean of expression from three uninfected mice were set at one) versus infected mice samples. Negative values represent levels of gene expression lower than those measured in naïve mice. Data are representative of two experiments and are obtained in groups of five mice per time points (mean ± SEM).