Figure 2.

Gating strategies for IL-17 producing cells. IL-17-fate reporter (Il17a^CreR26R^eYFP) and C57BL/6 mice were infected with 10⁵ P. chabaudi and sacrificed at days 3, 5, and 7 post-infection. (A) Representative flow cytometry plots of live cells gating in spleen and liver mononuclear cells. (B) Gating strategy for CD3⁺, CD4⁺, CD8⁺, NK⁺, and γδ⁺ T cells, and CD3⁻NK⁺ cells. (C) Total cell numbers of the several T cells populations in C57BL/6 splenocytes and liver mononuclear cells during the first 7 days of P. chabaudi infection. (D) Representative flow cytometry plots of IL-17^EYFP or IL-17A⁺ cells in CD4⁺ and CD8⁺ T cells of infected splenocytes and liver mononuclear cells of reporter and C57BL/6 mice, respectively.