Figure 3.

In vivo kinetics of IL-17A in spleen and liver of mice infected with P. chabaudi. IL-17-fate reporter (Il17a^CreR26R^eYFP) and C57BL/6 mice were infected with 10⁵P. chabaudi and sacrificed at days 3, 5, and 7 post-infection. (A) Total numbers of IL-17A^eYFP- and IL-17⁺-cells (detected by intracellular staining) in splenocytes and hepatocytes from naïve and infected IL-17-fate reporter mice (left panel) and C57BL/6 mice (right panel), respectively. The numbers represent the median and range of three mice. The fraction of CD4⁺, CD8⁺, γδ⁺, NK T cells, and NK cells contributing to IL-17A⁺ production in spleen and liver at day 7 and 5, respectively. (B) Representative flow cytometry plots of IL-17A^eYFP versus IFN-γ expression in CD4⁺ and CD8⁺ T cells, in liver cells, and splenocytes of naïve and day 5 and 7 infected mice. Numbers in quadrants refer to the percentages of cells in each quadrant. Data are representative of two experiments and are obtained in groups of three mice per time points (mean ± SEM).