Figure 3.

CD46 activation accelerates wound healing. (A) CD46 activation increases cell proliferation. Caco-2 cells were seeded into 24-wells at 1.5 × 10⁴ cells/well and allowed to adhere over night. Wells were then left untreated or treated with 10 μg/ml mAb to CD46, an isotype control mAb or staurosporine (200 μM) and cell proliferation measured 24 and 48 h post treatment. Data shown are the mean ± SD and representative of four experiments. (B) CD46 activation leads to accelerated wound closure. Caco-2 cells were cultured on 24-wells with a removable insert. After 6 days, the insert was removed and revealed a defined “wound-like” area (upper panels). mAbs to CD46 or an isotype control Ab was added and cell growth monitored via time-lapse microscopy. Lower panel shows the statistical analysis using MetMorph of the data obtained visually. Data shown are the mean ± SD and representative of three experiments. ns, statistically not significant.