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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Aging Cell. 2012 Feb 22;11(3):410–417. doi: 10.1111/j.1474-9726.2012.00796.x

Figure 4.

Figure 4

mKL gene transfer attenuated AngII-induced oxidative stress and apoptosis in RASM cells. A, Western blot bands of 4-HNE protein (lipid peroxidation product, oxidative stress marker) in RASM cells transfected with the klotho plasmid for 24 h followed by incubation with or without angiotensin II (200 nM) for 72 hours. B, Quantitative analysis of 4-HNE protein expression. C, Photomicrograph of 4-HNE staining in RASM cells. Arrows indicate 4-HNE-positive cells. D, Quantitative analysis of 4-HNE-positive cells. E, Quantitative analysis of apoptotic cells (Annexin V). Values=means±SEM. *p<0.05, **p<0.01, ***p<0.001 vs the LacZ group; ++p<0.01, +++p<0.001 vs the LacZ+AngII group. N=3 independent experiments with 3 measurements each.