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. Author manuscript; available in PMC: 2012 May 15.
Published in final edited form as: J Am Chem Soc. 2010 Nov 15;132(48):17247–17257. doi: 10.1021/ja107153a

Figure 7.

Figure 7

Internalization studies of FITC labeled octavalent anti-CD3 CSANs (green) to HPB-MLT T-leukemia cells. (a) Incubation at 37 °C or (b) 4 °C. (c) Colocalization with Alexa Fluor 594-labeled transferrin (red) after incubation at 37 °C. Incubation in the presence of clathrin-dependent endocytosis inhibitor chlorpromazine (d), actin polymerization inhibitor cytochalasin D (e), protein tyrosine kinase inhibitor genistein (f), caveolin-dependent endocytosis inhibitor nystatin (g), or cholesterol depletion agent methyl-β-cyclodextrin (h) at 37 °C. All incubations were for 30 min.