TABLE 1.

PPRE regions in the rat MAT2A promoter.

Name	Position	Matrix similarity score	PPRE region	Forward primer	Reverse primer
PPRE-1	−2061	0.928	TAGCCT-T-TGTCCC	5′-AATTCAAGGC-CAGTGAGTGAG-3′	5′-TCAATTCCTC-TGTCTTTAGCC-3′
PPRE-2	−1714	0.85	TAACCT-T-TTAATT	5′-CCTGTTAGTTA-ACAGTAGAATC-3′	5′-TCCAGCAGA-AAAATCTAT-3′
PPRE-3	−1656	0.774	TGACCA-G-TCTCCA	5′-GGCTTCCATA-TAGATTTTTCT-3′	5′-AGGCAGCATG-ATGATATT-3′
PPRE-4	−1433	0.894	TGAGCT-G-TGTCCC	5′-GAGCCATCTCTCTAGCTCCT-3′	5′-CTGTGGGAA-ACAAAGGAA-3′
PPRE-5	−1347	0.832	CCACCT-T-TGCACT	5′-AGGCTCCTAGCAAAGCCTAA-3′	5′-CTGAGAACCA-CCATTTGTCA-3′
PPRE-6	−364	0.806	TGGGCA-A-CGGTCG	5′-GTAGGATGAATAGGTGCGTG-3′	5′-GTGCCCCCTG-ACAGTAGTTA-3′

Six potential PPREs identified using the Transcription element search system (TESS) and MATInspector analysis tools are shown along with their sequence and the matrix similarity score. Only those PPREs with a matrix similarity score of approximately 0.8 or higher were selected for the study. The position of each PPRE is relative to the +1 start site of transcription. Sequences of the ChIP primers used to examine PPRE binding are shown.