Table 1.
Crystallographic summary.
| Parameter | Native |
|---|---|
| Space Group | P4132 |
| Dimensions (Å) a,b,c | 182.70 |
| X-ray source | APS 23ID-D |
| Wavelength λ (Å) | 0.97934 |
| dmin(Å) a | 2.90 (3.00–2.90) |
| Unique observations | 23,726 |
| Rmerge (%) a,b | 6.8 (57.8) |
| 〈I/σ〉 a | 27.7 (4.3) |
| Completeness (%) a | 99.9 (100) |
| Avg. redundancy a | 9.6 (9.7) |
| R/Rfree c,d | 0.238/0.265 |
| RMSD bond length (Å) | 0.007 |
| RMSD bond angle (°) | 0.757 |
| Avg. Protein B-factor (Å2) | 63.3 |
| Wilson B (Å2) | 78.8 |
| Ramachandran plot e | |
| Favored | 97.37 |
| Allowed | 2.37 |
| Disallowed | 0.26 |
a
Values in parenthesis are for outer shell
b
Rmerge = Σ|Ii-〈I〉|/ΣIi, where Ii is the intensity of the ith observation and 〈I〉 is the mean intensity
c
R = Σ|Fo-|Fc∥/Σ|Fo| where Fo is the observed structure factor and Fc is the calculated structure factor used in the refinement
d
Rfree = Σ|Fo-|Fc∥/Σ|Fo| where Fo is the observed structure factor and Fc is the calculated structure factor from 5% of reflections not used in the refinement
e
From MOLProbity62