Fig. 8.

Cbln4-null mice have no obvious neuroanatomical abnormalities. Representative matched coronal sections from wild type (A–C) or Cbln4-null (D–E) mice were stained with hematoxylin and eosin. The sections were selected to highlight regions of brain (boxes in A–F) that have anomalies in netrin- and DCC-null mice. Compared with wild-type (A–C) mice, Cbln4-null mice (D–F) show no overt neuroanatomical defects. Furthermore, specific structures such as corpus callosum (CC in D, E), hippocampal commissure (HC, in E), and pontine nuclei (PN in F) that are lacking in netrin- and DCC-null mice are present and indistinguishable from wild type littermates in Cbln4-null mice. Scale bar, 750 μm for A–F and 100 μm for the insets.