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. Author manuscript; available in PMC: 2013 May 2.
Published in final edited form as: J Am Chem Soc. 2012 Apr 23;134(17):7215–7218. doi: 10.1021/ja3003606

Figure 4.

Figure 4

Slices are shown from the NCACX and NCOCX regions of a 3D ZF-TEDOR-RFDR spectrum used for sequential assignments. For P25, the NCδ transfer was also efficient (second panel). 1.2 ms of TEDOR mixing and 4.8 ms of RFDR mixing with 83 kHz pulses were used. The direct acquisition used a 6 μs dwell time and 3072 points (~18.4 ms), the indirect 13C dimension was acquired with a 50 μs dwell time and 180 complex points (9 ms), and the 15N dimension used a 150 μs dwell time and 74 complex points (11.1 ms). The carrier frequency was set to 100 ppm for 15N, and 89 ppm for 13C. The spectrum was acquired in ~5 days and provided useful NCACX and NCOCX connectivity information. The sample temperature was ~30 °C, and the spinning frequency was 20 kHz.