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Western blot analysis of ETHE1 expression in murine liver, kidney and brain. Tissue extracts (100 μg of protein/lane) was separated on a denaturing 12% polyacrylamide gel and detected with anti-human ETHE1 antibody as described under Methods. A sample of purified recombinant human ETHE1 (5 ng) lacking the mitochondrial leader sequence was used as a positive control. The positions of molecular weight standards at 25, 37, and 50 kDa are indicated.
