Table 1.
Disappearance of H2S Under Aerobic and Anaerobic Conditions
| Sample/conditions | Percent H2S left after 20 min incubation (mean±SD) | Number of experiments | Statistical significance |
|---|---|---|---|
| Aerobic buffer | 84.8±5.1 | 6 | The difference between all the buffer data is not significant p>0.1 |
| Anaerobic buffer | 81.4±2.8 | 7 | |
| “Strictly” anaerobic buffer | 82.7±2.2 | 7 | |
| “Strictly” anaerobic kidney homogenate | 100.1±7.9 | 3 | The difference between kidney and all the buffer data is significant p<0.01 |
| “Strictly” anaerobic liver homogenate | 51.8±21.5 | 6 | The difference between all the liver data is not significant p>0.1 The difference between all the liver and buffer data is significant p<0.02 |
| “Strictly” anaerobic liver homogenate flushed with CO | 63.1±12.8 | 3 | |
| “Strictly” anaerobic liver homogenate+2 mM DTT | 71.0±8.9 | 4 |
H2S levels were monitored after 20 min at 25°C, in the following samples: aerobic buffer, anaerobic buffer, buffer in an anaerobic chamber (“strictly” anaerobic), kidney homogenate in anaerobic chamber, liver homogenate in anaerobic chamber, liver homogenate flushed with CO in anaerobic chamber, liver homogenate+2 mM DTT in anaerobic chamber.