Figure 5. In vitro cytokine production by primary microglia cultures derived from either wild-type or UNC93b1-mutant mice.

Cultures were prepared as outlined in Materials and Methods section. Three wells were left untreated, exposed to NSV or stimulated with synthetic ligands for the TLRs indicated also as described in Materials and Methods section. Cytokine concentrations were measured in culture supernatants 24 h later using ELISA-specific for IL-12p40 (A), TNFα (B) and IFN-α (C). The production of these mediators was measured using primary cells prepared on two separate occasions.