Figure 7. Effects of Baf A1, a pharmacological inhibitor of virus uncoating in endosomes, on cytokine and chemokine production by primary microglia cultures prepared from wild-type mice and then exposed to NSV.

Some wells were treated with Baf A1 as outlined in Materials and Methods section. A minimum of three wells either without or with Baf A1 pretreatment were then exposed to NSV, and cytokine and chemokine concentrations measured by ELISA in culture supernatants 24 h later using ELISA-specific for IL-12p40 (A), IFN-α (B), CCL2 (C) and CCL5 (D). The production of these mediators without or with Baf A1 treatment was measured using primary cells prepared on two separate occasions.