Table 1.

Primer sequences for polymerase chain reaction

Gene	Primer sequences (forward/reverse 5’-3’)	Accession No.	Location to transcription start	Product size (bp)	Annealing temperature (°C)
SPARC methylation	GAGAGCGCGTTTTGTTTGTC	NM_003118.2	+52 to +71	112	54
	AACGACGTAAACGAAAATATCG		+142 to +163
SPARC unmethylation	TTTTTTAGATTGTTTGGAGAGTG	NM_003118.2	+36 to +58	132	59
	AACTAACAACATAAACAAAAATATC		+143 to +167
SPARC BS	GATAGAGATAGTTTTGGTTATGGGA	NM_003118.2	-119 to -95	401	55
	CCACCTTCTAAAAAACA ACAAAC		+260 to +282
SPARC mRNA	CGCATGCGGGACTGGCTCAA	NM_003118.2	+601 to +620	148	60
	GCTCCACGGGG TGGTC TCCT		+729 to +748
GAPDH mRNA	GGGCATCCTGGGCTACACTGA	NM_002046.3	+915 to +935	143	58
	CAAATTCGTTGTCATACCAGGAAATG		+1032 to +1057

SPARC: Secreted protein acidic and rich in cysteine; BS: Bisulfite sequencing; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase.