Table 3.

Summary of DNA vaccination protocols

Animal ID	Age	Immunogen	Dose and route	Comments	Boost
A. H636, H642, H615	Adult	vapA + IL-12 (1 mg each, mixed)	0.5 mg i.d. 0.5 mg i.t.	i.d. divided between 10 sites i.t. single injection	Day 14—same protocol as primary
B. H607, H624, H563	Adult	vapA + control plasmid	Same as (A)	Same as (A)	Same as (A)
C. H670, H669, H672, H673	Foals	vapA + IL-12 (1 mg each, mixed)	0.5 mg i.d. 0.5 mg i.n.	i.d. divided between 10 sites i.n. one dose Prime-Boost*	Day 14—same (DNA) Day 30—rVapA protein*: i.d. 10 μg × 10 sites (100 μg total) + Ribi™ adjuvant; +100 μg protein i.n.
D. H629, H633, A2166, JJ, MM	Foals	vapA plasmid [20]	Same as (C)	Same as (C)	Same as (C)
E. H630, H632, A2167, JH, Elly	Foals	vapA control plasmid [20]	Same as (C)	Same as (C)	Same as (C)
F. A2201, A2185	Adult	p15/p26 + IL-12 (5 mg each, mixed)	2.5 mg i.d. 2.5 mg i.t.	i.d. divided between 10 sites i.t. single injection	Day 15, 43–57, 57–71
G. A2190, A2192	Adult	p15/p26 + control	Same as (F)	Same as (F)	Same as (F)
H. A2222, A2223, A2224	Foals	p15/p26 alone (no IL-12)	Same as (F)	Same as (F)	Day 15, 36, 50

^*DNA prime + boost with recombinant protein.