Figure 4.

Characterization of G183A-mediated uridine uptake. (A) TUBA5 parasites expressing WT (●), G183A (▴), or vector pX63Hyg (□) were assayed for 1 μM [³H]uridine uptake. Results are expressed as mean ± SD (n = 2). (B) Uptake of [³H]adenosine (250 nM) by TUBA5 parasites expressing WT or G183A transporters was determined over a 10-s period in the absence or presence of uridine (40 μM). Results are expressed as the percentage of uptake in the absence of uridine, and each value is the mean ± SD (n = 3).