Table 1.
Kinetic parameters for WT and mutant transporters
| Km, μM | Vmax, pmol/108 cells/s | Vnmax, pmol/108 cells/s | |
|---|---|---|---|
| WT | 0.38 ± 0.02 | 3.24 ± 0.22 | 3.24 ± 0.22 |
| G183D | 2.3 ± 0.45 | 0.09 ± 0.008 | 0.18 ± 0.006 |
| C337Y | 1.99 ± 0.3 | 0.22 ± 0.014 | 0.14 ± 0.008 |
Substrate saturation curves for adenosine were obtained for TUBA5 parasites expressing GFP-WT, GFP-G183D, and GFP-C337Y as described in Materials and Methods. Each value is the mean ± SD of two independent determinations. V
n
max values are Vmax values normalized to protein levels determined by Western blot analysis as described in the text.