Figure 3.

The characteristic of cerebellum neurons isolated from the brain of animals after prenatal hyperhomocysteinemia not treated and treated with pinealon (typical record). Isolated cerebellum granule cells were loaded with DCFH₂ and PI and then analyzed by flow cytometry technique as described in Materials and methods. Axis FL1 corresponds to DCF fluorescence, marker of free radical levels, abscissa; FL2 – to PI fluorescence, marker of necrotic cells, ordinate). A and B correspond to the intact cells isolated from the pups of methionine loaded rats not treated (A) or treated (B) with pinealon, C and D – the same as A and B after the 30 min incubation of the isolated cells with 5 mM H₂0₂.