Table 1. Summary of the Relative Intensities of Selected DGGE Excised Bands for Every Duplicate DNA Extraction and Their Average for Different Treatments and Time Point .
N7 DGGE Mycobacterium-like sequence |
N8 DGGE Rhodococcus RHA1-like
sequence |
N23 DGGE Rhodococcus erythropolis-like
sequence |
||||||
---|---|---|---|---|---|---|---|---|
relative
band intensity % |
relative band intensity % |
relative band
intensity % |
||||||
soil | time | sample | average | average | average | |||
unamended | 0 | DNA1 | 1.87 | 1.52 ± 0.50 | 2.93 | 2.72 ± 0.30 | 1.59 | 1.43 ± 0.22 |
DNA2 | 1.16 | 2.51 | 1.28 | |||||
6 months | DNA1 | 4.50 | 4.46 ± 0.05 | 3.26 | 3.30 ± 0.06 | 1.84 | 1.87 ± 0.04 | |
DNA2 | 4.42 | 3.34 | 1.90 | |||||
3 years | DNA1 | 3.00 | 2.96 ± 0.06 | 8.36 | 8.92 ± 0.80 | 2.21 | 2.35 ± 0.20 | |
DNA2 | 2.92 | 9.48 | 2.50 | |||||
PAC | 0 | DNA1 | 3.48 | 3.15 ± 0.46 | 4.65 | 4.26 ± 0.55 | 2.22 | 2.16 ± 0.08 |
DNA2 | 2.83 | 3.87 | 2.11 | |||||
6 months | DNA1 | 3.42 | 3.04 ± 0.54 | 3.34 | 3.33 ± 0.004 | 1.94 | 2.00 ± 0.08 | |
DNA2 | 2.65 | 3.33 | 2.06 | |||||
3 years | DNA1 | 3.25 | 3.49 ± 0.34 | 9.37 | 9.26 ± 0.16 | 3.32 | 3.03 ± 0.40 | |
DNA2 | 3.73 | 9.14 | 2.74 | |||||
GAC | 0 | DNA1 | 1.96 | 1.84 ± 0.17 | 3.53 | 3.19 ± 0.50 | 1.66 | 1.57 ± 0.12 |
DNA2 | 1.71 | 2.85 | 1.48 | |||||
6 months | DNA1 | 4.11 | 2.64 ± 2.08 | 3.33 | 2.71 ± 0.89 | 2.11 | 1.82 ± 0.40 | |
DNA2 | 1.17 | 2.08 | 1.54 | |||||
3 years | DNA1 | 3.73 | 3.34 ± 0.55 | 8.55 | 7.90 ± 0.92 | 2.77 | 2.67 ± 0.15 | |
DNA2 | 2.96 | 7.25 | 2.57 |