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PCR primers and temperature regime.
| Mammalian specific primers 5’-CGAAGCTTGATATGAAAAACCATCGTTG-3’(f) 5’-TGTAGTTRTCWGGGTCHCCTA-3’ (r) |
Avian-specific primers 5’-GACTGTGACAAAATCCCNTTCCA-3’(f) 5’-GGTCTTCATCTYHGGYTTACAAGAC-3’(r) |
|||
|---|---|---|---|---|
| Initial Denaturation | 10 min 95° C | 10 min 95° C | ||
| 36 cycles |  |
Denature | 30 sec 95° C | 30 sec 95° C |
| Anneal | 40 sec 55° C | 40 sec 56° C | ||
| Extension | 50 sec 72° C | 50 sec 72° C | ||
| Final extension | 3 min 72° C | 3 min 72° C | ||
