Table 2.

Expression of selected epidermal, BMZ and dermal genes in zebrafish at different stages of development ¹⁾

Gene	Developmental stage
	1 dpf	6 dpf	adult
Epidermal
krt1	−	+	+
krt5	−	+	++
bpag1	−	++	+
plec1	+	+	++
BMZ
col4a1	+	+	++
col4a2	−	++	+
col4a3	−	+	+
col4a4	−	+	+
col4a5	−	−	−
col4a6	++	++	+
col7a1a	+	+	+
col7a1b	+	+	+
col17a1a	−	+	+
col17a1b	+	++	+
itga6	−	+	++
itgb4	+	++	+++
Dermal
col1a1	+	++	+++
col1a2	−	++	+
col1a3	+	++	+++
col5a1	+++	++	+
col5a2	−	−	−
col6a1	+	+	−
col6a2	−	+	+
col12a1	−	+	++
col14a1	−	+	+
col15a1	−	++	+
col16a1	−	+	+
col18a1	+	++	+++
col19a1	++	+	+
Reference gene
β-actin	+	+	+

¹⁾Total RNA was isolated from developing zebrafish at 1 and 6 dpf, as well as from an ~ 2-year old adult. The presence of gene expression was determined by RT-PCR with specific primers using 35 cycles. The presence (+) or absence (−) of the PCR product was assessed by 2% agarose gel electrophoresis. The relative amount of each PCR product at different developmental stages was semi-quantitatively indicated by +, ++ or +++.