Figure 5.

Stability of mRNA for IL-6 with H₂O₂ treatment in HTM cells grown on 1400-nm pitch topography compared with planar surfaces. The result of one representative culture of two, obtained from a 26-year-old donor, is shown. HTM cells were treated with 5 μg/mL actinomycin D to suppress transcription (t = −30 minutes). Data are shown as mean ± standard deviation of the triplicate and normalized to t = −30 minutes of planar or topographic surfaces. No difference in stabilization of mRNA was noted between the cells on topography and planar substrates. Although nanopatterned surfaces influence a number of cellular behaviors, nanopatterned surfaces did not alter IL-6 mRNA stability. This result suggests topographic cues do not affect ARE stabilization by HuR.