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. Author manuscript; available in PMC: 2012 May 3.
Published in final edited form as: Nature. 2011 Sep 11;477(7365):424–430. doi: 10.1038/nature10495

Figure 5. IGCR1 mediates long distance IgH chromosomal loops.

Figure 5

(a) Schematic of chromosome interactions between IGCR1-containing and 3′IgHCBE-containing KpnI restriction fragments in 3C assays. Interactions between IGCR1 and 3′IgHCBE locales in 129SV RAG2-/- and RAG2-/-IGCR1-/- A-MuLV transformed pro-B cells were quantified by real time PCR (Taqman) using probe (P2) (left) and a probe (P1) (right). (b) Schematic of chromosome interactions between iEμ-containing KpnI restriction fragment and indicated KpnI restriction fragments in other IgH locales. Interactions between iEμ and IGCR1, iEμ and 3′IgHRR locales, iEμ and 3′IgHCBE locales in RAG2-/- and RAG2-/-IGCR1-/- A-MuLV transformed pro-B cells were quantified by real time PCR using a probe (P3) from the iEμ locale. F1-F8 indicate primers used for PCR. K indicates KpnI sites. Red arcs indicate interactions detected in RAG2-/- cells. The average association frequency of three independent 3C experiments with 2 independent A-MuLV transformed lines from each genotype is shown with standard deviation indicated.