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. Author manuscript; available in PMC: 2013 May 1.

Published in final edited form as: Hepatology. 2012 Mar 18;55(5):1585–1595. doi: 10.1002/hep.24802

Fig. 5 — RAW macrophages were transfected with either NFκB (A) or TNFα (B) promoter constructs separately. Next day the cells were stimulated with LPS ( 100 ng/ml), 17-DMAG ( 0.5 µM) or both () for 6 hrs. Fold induction in NFκB (A) and TNFα (B) promoter activity over unstimulated cells is shown as bar graph. *p < 0.05 vs. unstimulated cells, ^#p < 0.001 vs. LPS stimulated cells. Data represents mean of 3 experiments ± SEM. Chromatin immunoprecipitation assay was performed using anti-HSF1 antibody and semi quantitative PCR was carried out using hsp70 (C), TNFα (D), and IL-6 (E) promoter specific primers. A representative gel picture for each gene is shown. The densitometry graph represents average of 3 independent experiments. *p < 0.0001 vs. LPS stimulated cells; #p < 0.001 vs. unstimulated cells.

Fig. 5 — RAW macrophages were transfected with either NFκB (A) or TNFα (B) promoter constructs separately. Next day the cells were stimulated with LPS ( 100 ng/ml), 17-DMAG ( 0.5 µM) or both () for 6 hrs. Fold induction in NFκB (A) and TNFα (B) promoter activity over unstimulated cells is shown as bar graph. *p < 0.05 vs. unstimulated cells, ^#p < 0.001 vs. LPS stimulated cells. Data represents mean of 3 experiments ± SEM. Chromatin immunoprecipitation assay was performed using anti-HSF1 antibody and semi quantitative PCR was carried out using hsp70 (C), TNFα (D), and IL-6 (E) promoter specific primers. A representative gel picture for each gene is shown. The densitometry graph represents average of 3 independent experiments. *p < 0.0001 vs. LPS stimulated cells; #p < 0.001 vs. unstimulated cells.