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. Author manuscript; available in PMC: 2012 May 3.
Published in final edited form as: Stat Appl Genet Mol Biol. 2012 Mar 31;11(3):10.1515/1544-6115.1750 /j/sagmb.2012.11.issue-3/1544-6115.1750/1544-6115.1750.xml. doi: 10.1515/1544-6115.1750

Figure 1. ChIP-seq and MeDIP-seq.

Figure 1

Chromatin is randomly sheared with high frequency sound waves (sonication) or digested with micrococcal nuclease (MNase). The desired Protein:DNA complex is then isolated with an antibody (yellow Y). The ends of purified DNA are then sequenced (ChIP-seq). Similarly, MeDIP-seq uses an antibody against methyl cytosine, followed by deep sequencing. Mapping the resulting short reads to the reference human genome then provides information about which genomic loci were modified or bound by a TF.