Table 1.
Fold-change of gene expression associated with primitive hematopoiesis in metap2MO embryos at 18 hpf
| Gene | Control | metap2MO | P value |
|---|---|---|---|
| scl | 1.00 | 0.89 ± 0.07 | .17 |
| lmo2 | 1.00 | 0.92 ± 0.08 | .15 |
| gata1 | 1.00 | 1.24 ± 0.07 | .17 |
| ehα1 | 1.00 | 1.14 ± 0.12 | .43 |
| ehβ1 | 1.00 | 1.18 ± 0.08 | .25 |
| pu.1 | 1.00 | 1.24 ± 0.05 | .07 |
| l-plastin | 1.00 | 0.99 ± 0.09 | .25 |
| mpo | 1.00 | 2.06 ± 0.10 | .02 |
Comparison of gene expression between control and the metap2MO embryos in each case was based on ΔCt as described previously. Statistical analysis was evaluated by the Student paired t test. Data as shown represent the average results of 3 experiments.