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. 2012 Apr 17;4(4):602–616. doi: 10.1093/gbe/evs041

Table 1.

Primers Used in This Study

Name Species Template Primer Sequence
AcORg_F1 Anolis carolinensis Genomic DNA GGGCTCTGAGATGGCATATGAYCGVTAYKTKGC
AcORg_R1 A. carolinensis Genomic DNA GGGCTGTCAGGAACAGGTRGARAARGCYTT
AcORm_F1 A. carolinensis Nose cDNA GGGCTCGTAGATGGCATATGAYCGVTAYKTKGC
AcORm_R1 A. carolinensis Nose cDNA GGGCTGTACGGAACAGGTRGARAARGCYTT
EqORg_F1 Elaphe quadrivirgata Genomic DNA GGGCTCGATGATGGCATATGAYCGVTAYKTKGC
EqORg_R1 E. quadrivirgata Genomic DNA GGGCTGCTAGGAACAGGTRGARAARGCYTT
F2 A. carolinensis Genomic DNA ATGGCATATGAYCGVTAYNTDGC
R2 A. carolinensis Genomic DNA GAACAGGTDGARARWGYYTT
F3 A. carolinensis Genomic DNA CATATGAYCGVTAYKTDGCYATHTG
R3 A. carolinensis Genomic DNA CAGTAARRTGGGARSHRCADGTDGA

NOTE.—The first six primers were used for the NGS experiments, whereas the other four primers were used for manual sequencing of clones for amplified products. Tag sequences are indicated by underlines. Note that three forward primers ending in F1 share identical sequences after the tag sequences (the F1 primer sequence) and that three reverse primers ending in R1 do so (the R1 primer sequence). F1–F3 primers are forward primers, and R1–R3 primers are reverse ones. F2 and R2 primers are designed in the same location as F1 and R1 primers, respectively, but have slightly different bases in some positions. F3 and R3 primers are designed in different locations, but the amplified F3–R3 region largely overlaps with the F1–R1 (= F2–R2) region.