Table 1.

Primers Used in This Study

Name	Species	Template	Primer Sequence
AcORg_F1	Anolis carolinensis	Genomic DNA	GGGCTCTGAGATGGCATATGAYCGVTAYKTKGC
AcORg_R1	A. carolinensis	Genomic DNA	GGGCTGTCAGGAACAGGTRGARAARGCYTT
AcORm_F1	A. carolinensis	Nose cDNA	GGGCTCGTAGATGGCATATGAYCGVTAYKTKGC
AcORm_R1	A. carolinensis	Nose cDNA	GGGCTGTACGGAACAGGTRGARAARGCYTT
EqORg_F1	Elaphe quadrivirgata	Genomic DNA	GGGCTCGATGATGGCATATGAYCGVTAYKTKGC
EqORg_R1	E. quadrivirgata	Genomic DNA	GGGCTGCTAGGAACAGGTRGARAARGCYTT
F2	A. carolinensis	Genomic DNA	ATGGCATATGAYCGVTAYNTDGC
R2	A. carolinensis	Genomic DNA	GAACAGGTDGARARWGYYTT
F3	A. carolinensis	Genomic DNA	CATATGAYCGVTAYKTDGCYATHTG
R3	A. carolinensis	Genomic DNA	CAGTAARRTGGGARSHRCADGTDGA

NOTE.—The first six primers were used for the NGS experiments, whereas the other four primers were used for manual sequencing of clones for amplified products. Tag sequences are indicated by underlines. Note that three forward primers ending in F1 share identical sequences after the tag sequences (the F1 primer sequence) and that three reverse primers ending in R1 do so (the R1 primer sequence). F1–F3 primers are forward primers, and R1–R3 primers are reverse ones. F2 and R2 primers are designed in the same location as F1 and R1 primers, respectively, but have slightly different bases in some positions. F3 and R3 primers are designed in different locations, but the amplified F3–R3 region largely overlaps with the F1–R1 (= F2–R2) region.