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. 2001 May 15;98(11):6104–6109. doi: 10.1073/pnas.111382798

Figure 1.

Figure 1

Purification and functional characterization of recombinant RyR3 and GST-RyR3. (A) Twenty-five microliters of purified recombinant RyR3 (glutathione-eluted) and GST-RyR3 were subjected to a 12% SDS/PAGE gel and stained with Coomassie brilliant blue. (B) A Western blot of 2 μl of purified RyR3 and GST-RyR3 by using the anti-RyR antibody 34C (Affinity BioRegents). (C) HEK293 cells were transfected with 10 μg of RyR3 or GST-RyR3 cDNA. Fluorescence intensity of fluo 3-loaded cells was measured before and after additions of 2.5 mM caffeine (●) and 50 μM ryanodine (○).